testingmethodsop

 PURPOSE: To describe the Laboratory standard operating procedure for measurement of Biological Oxygen Demand of Water and Waste Water samples. SCOPE: Applicable to the Laboratory for analysis of Water and Waste Water samples, where measurement of Biological oxygen demand is required.   RESPONSIBILITY:  Lab chemist: Analysis of sample for measurement of Biological Oxygen demand. Technical Manager:   Review of activity  Quality Manager: Implementation and compliance of SOP. PROCEDURE:  Principle: Biochemical Oxygen Demand (BOD) is defined as the amount of O2 required by microorganisms while stabilizing biologically decomposable organic matter in a waste under aerobic conditions. Instrument and Equipment: BOD bottles 300ml capacity, BOD Incubator to be controlled at 27oC ± 1oC. Reagents:  Phosphate buffer: Dissolve 8.5 g KH2PO4, 21.75 g K2HPO4, 33.4 g Na2HPO4.7H2O and 1.7 g NH4Cl in distilled water and dilute to 1000ml. Adjust its pH to 7.2. Magnesi...