testingmethodsop

 Purpose: Todescribe the Laboratory Standard Operating Procedure for measurement of Ammonical Nitrogen of Water and Waste Water samples.  Scope:  Applicable to the Laboratory for analysis of water and waste water samples, where measurement of Ammonical Nitrogen is required.  Responsibility: Lab Chemist:  Analysis of sample for measurement of Ammonical Nitrogen.  Technical Manager: Review of Activity Quality Manager: Implementation and compliance of SOP.  Procedure: 1) Principle: The sample is buffered at pH 9.5 with borate buffer to decrease hydrolysis of cyanates and organic nitrogen compounds. It is then distilled into boric acid. The ammonia in the distillate is then determined titrimetrically with standard sulfuric acid or calorimetrically.  2) Instrument and Equipment: Pipettes, Conical flask, Nitrogen distillation assembly, Heating Mantle.  3) Reagents: • Sodium tetraborate 0.025M: Prepare by dissolving 9.5 g Na2B4O7. 10H2O in distilled...

  SOP For Total Hardness in Water and waste water EDTA Titrimetric method Purpose: The purpose of this protocol is to provide guidelines for analysis of Total Hardness in Water. Principle of method: EDTA and it's sodium salts from a chelated solution complex when added to a solution of certain metal actions, if a small amount of dye such as Eriochrome Black T is added to an aqoeous solution containing calcium and magnesium ions at a pH of 10+0.1 to 10-0.1, the solution becomes winr red. When titrated with EDTA , the magnesium and calcium will be complexed, and when all of the  and  has been complexed the solution turns from wine red to Blue.      To   Instrument/Equipment: The following items are necessary to perform the analysis of Total Hardness in Water; pH Meter Burette – 50 ml capacity Glass Wares Reagents/Chemicals: All the chemicals should be of Analytical Reagent Grade. Distilled Water Buffer Solution – Dissolve 16.9 g of Ammonium Chloride...

  PURPOSE: To describe the Laboratory standard operating procedure for measurement of Oil & grease in Water and Waste Water samples. SCOPE: Applicable to the Laboratory for analysis of Water and Waste Water samples, where measurement of Oil & grease is required.   RESPONSIBILITY:  Lab chemist: Analysis of sample for measurement of Oil & grease. Technical Manager Review of activity  Quality Manager: Implementation and compliance of SOP. PROCEDURE:  Instrument and Equipment: Analytical Balance  . Separator Funnel 1000 ml. Beaker 150 ml  Measuring Cylinder, 100 ml. Whatman Filter Paper No.1 Reagents:  Sulphuric Acid (1:2, diluted) Petroleum ether (40o – 60o C, Boiling Range) Sodium Sulphate anhydrous. Test Method: Take 500 ml of sample in a separator funnel. Add 10 ml of Sulphuric Acid (1+2). Add 40 ml of Petroleum Ether. Shake vigorously for two minutes and keep for some time to separate the two distinct layers, the upper one of Petroleu...

 PURPOSE: To describe the Laboratory standard operating procedure for measurement of Chemical Oxygen Demand of Water and Waste Water samples. SCOPE: Applicable to the Laboratory for analysis of Water and Waste Water samples, where measurement of Chemical Oxygen Demand is required.   RESPONSIBILITY:  Lab chemist: Analysis of sample for measurement of Chemical Oxygen Demand. Technical Manager: Review of activity  Quality Manager: Implementation and compliance of SOP. PROCEDURE:  Principle: A sample is refluxed in strong acidic solution with a known excess of potassium dichromate (K2Cr2O7).After digestion the remaining unreduced K2Cr2O7 is titrated with ferrous ammonium sulphate to determine the amount of potassium dichromate (K2Cr2O7) consumed and the oxidizable matter is calculated in terms of oxygen equivalent.  Instrument and Equipment: Heating Mantle, Burette 50 ml. Round Bottom Flask 500 ml Pipettes 50ml. Measuring Cylinders Reagents:  Preparation o...

 PURPOSE: To describe the Laboratory standard operating procedure for measurement of Dissolved Oxygen of Water and Waste Water samples. SCOPE: Applicable to the Laboratory for analysis of Water and Waste Water samples, where measurement of Dissolved oxygen is required.   RESPONSIBILITY:  Lab chemist : Analysis of sample for measurement of Dissolved oxygen. Technical Manager : Review of activity  Quality Manager : Implementation and compliance of SOP. PROCEDURE:  Principle: Oxygen present in sample oxidize the divalent Mn to its high valency which precipitates as a brown hydrate oxide after addition of sodium hydroxide and KI. Upon acidification, manganese reverts to divalent state and liberates iodine from potassium iodideiodidel equivalent to DO content in the sample. The liberated iodine is titrated against 0.025N Na2S2O3 using starch as indicator. Instrument and Equipment: BOD bottles 300ml capacity, 25 ml Burette  Reagents:  Magnus sulfate: Dissolv...