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Standard Operating Procedure for the Measurement of Oil & Grease

 


PURPOSE:

To describe the Laboratory standard operating procedure for measurement of Oil & grease in Water and Waste Water samples.





SCOPE:

Applicable to the Laboratory for analysis of Water and Waste Water samples, where measurement of Oil & grease is required.

 

RESPONSIBILITY: 

Lab chemist:

Analysis of sample for measurement of Oil & grease.

Technical Manager


Review of activity 

Quality Manager:

Implementation and compliance of SOP.


PROCEDURE: 

Instrument and Equipment:

Analytical Balance 

.

Separator Funnel 1000 ml.

Beaker 150 ml 

Measuring Cylinder, 100 ml.

Whatman Filter Paper No.1

Reagents: 

Sulphuric Acid (1:2, diluted)

Petroleum ether (40o – 60o C, Boiling Range)

Sodium Sulphate anhydrous.

Test Method:

Take 500 ml of sample in a separator funnel.

Add 10 ml of Sulphuric Acid (1+2).

Add 40 ml of Petroleum Ether.

Shake vigorously for two minutes and keep for some time to separate the two distinct layers, the upper one of Petroleum Ether and the lower one of the sample.

Collect the lower aqueous layer in a clean beaker.

Filter the Petroleum Ether layer through funnel with a whatman filter paper no.1.

containing about 10 gm crystals of anhydrous Na2SO4, to remove the traces of

moisture from the Ether layer into a previously weighed (W1 gm.) clean, dry 150 ml beaker.

Transfer quantitatively the aqueous layer back to the separator funnel.

Rinse the beaker twice with 10 ml Petroleum Ether every time and transfer the ether washing to the aqueous layer in separating funnel.

Repeat the above step

Discard the aqueous layer.

Wash the filter paper and crystals of Na2SO4 , twice with about 10 ml of Ether 

Collect the Ether washing in a previously collected filtrate of Petroleum Ether.

Evaporate the total Petroleum Ether extract, collected in a beaker, on a water bath.

Dry the residue of Oil & Grease obtained in an oven at 80°C.

Cool the dried residue in a desiccators and Weigh. (W2 gm.) 

5. Calculation:

Oil & Grease (mg/L) =        (W2 – W1) X1000 x 1000 

                                                      --------------------------------------

                                                          Volume of Sample taken


RECORDS: 

Analyst Register and Reports




EHS Instructions (Wherever applicable):


     1:  Use gloves while handling acids, alkali, solvents & Hazardous waste .


     2:  Handle all glassware with care to avoid OHS incident.


     3:  Use of apron wherever necessary


     4. Avoid spillage of chemical / waste sample


 5. Take precaution to avoid the fire incidents.


      6. Ensure the disposal of Hazardous waste sample.




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